Free shipping starts now, no minimum, no coupons required!

Anti-Human ZACN (extracellular) Antibody

Zinc-activated ligand-gated ion channel, Ligand-gated ion channel zinc-activated 1, Ligand-gated ion-channel receptor L2, ZAC, LGICZ1
Cat #: AZC-001
Alternative Name Zinc-activated ligand-gated ion channel, Ligand-gated ion channel zinc-activated 1, Ligand-gated ion-channel receptor L2, ZAC, LGICZ1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h
Immunogen
  • Peptide CNFELLHFPRDHSN, corresponding to amino acid residues 157-170 of human ZACN (Accession Q401N2). Extracellular, N-terminus.
Accession (Uniprot) Number Q401N2
Gene ID 353174
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Human.
RRID AB_11219142.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ifc, lci, wb
May also work in: ih*, ip*
Western blot
  • Western blot analysis of PANC-1 (lanes 1 and 3) and Jurkat (lanes 2 and 4) cell lysates:
    Western blot analysis of PANC-1 (lanes 1 and 3) and Jurkat (lanes 2 and 4) cell lysates:
    1,2. Anti-Human ZACN (extracellular) Antibody (#AZC-001), (1:200).
    3,4. Anti-Human ZACN (extracellular) Antibody, preincubated with Human ZACN (extracellular) Blocking Peptide (#BLP-ZC001).
Indirect flow cytometry
  • Cell surface detection of ZAC in Jurkat living cells:
    Cell surface detection of ZAC in Jurkat living cells:
    ___ Unstained cells.
    ___ Cells + Anti-Human ZACN (extracellular) Antibody (#AZC-001), (5-10 µg/1x106 cells).
  • The blocking peptide is not suitable for this application.
Live cell imaging / Immunocytochemistry
  • Expression of Zinc-activated channel in human PANC-1 cells
    Expression of Zinc-activated channel in human PANC-1 cells
    Cell surface detection of Zinc-activated channel in human intact living pancreatic adenocarcinoma (PANC-1) cells. Extracellular staining (green) of cells with Anti-Human ZACN (extracellular) Antibody (#AZC-001), (1:25) followed by goat anti-rabbit-AlexaFluor-488 secondary antibody. DAPI is used as the counterstain (blue).
References
  1. Davies, P.A. et al. (2003) J. Biol. Chem. 278, 712.
  2. Houtani, T. et al. (2005) Biochem. Biophys. Res. Comm. 335, 277.
Scientific background

Over forty homologous proteins belong to the superfamily of ligand-gated ion channels. These members are divided into subfamilies, for example: acetylcholine, serotonin and glycine receptors.

A new member has been added to this superfamily – ZAC (Zinc-activated channel) which is expressed in humans and dogs but not in rodents1. Like all other ligand-gated ion channels, ZAC has four transmembrane domains and extracellular N- and C-termini. As its name states, the channel is activated by high concentrations of zinc.

ZAC mRNA was found to be expressed in the placenta, spinal cord, stomach, and fetal brain1. In another study, ZAC was identified using a computer-assisted program and was detected in similar areas (including pancreas and kidney), but also displayed minor discrepancies2.

Being that the channel is expressed in human and dog, deciphering its cellular/physiological functions will be challenging.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 23/08/2020

Anti-Human ZACN (extracellular) Antibody (#AZC-001) is a highly specific antibody directed against an epitope of the human Zinc-activated ligand-gated ion channel. The antibody can be used in western blot, immunocytochemistry, and indirect flow cytometry applications. The antibody recognizes an extracellular epitope, and is thus ideal for detecting ZAC in living cells. It has been designed to recognize ZAC from human samples only.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Shipping and Ordering information