Anti-Human Zinc-Activated Channel (ZAC) (extracellular) Antibody

Ligand-gated ion channel zinc-activated 1, Ligand-gated ion-channel receptor L2, ZACN
    Cat #: AZC-001
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h
    Immunogen
    Peptide CNFELLHFPRDHSN, corresponding to amino acid residues 157-170 of human zinc-activated channel (ZAC) (Accession Q401N2). Extracellular, N-terminus.
    Accession (Uniprot) Number Q401N2
    Gene ID 353174
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Human.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20ºC.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, ifc, lci, wb
    May also work in: ih, ip
    Western blot
    Western blot analysis of Panc-1 (lanes 1 and 3) and Jurkat (lanes 2 and 4) cell lysates:
    1, 2. Anti-Human Zinc-Activated Channel (ZAC) (extracellular) Antibody (#AZC-001), (1:200).
    3, 4. Anti-Human Zinc-Activated Channel (ZAC) (extracellular) Antibody, preincubated with the control peptide antigen.
    Indirect flow cytometry
    Indirect flow cytometry analysis of Jurkat living cells:
    ___ Unstained cells.
    ___ Cells + Anti-Human Zinc-Activated Channel (ZAC) (extracellular) Antibody (#AZC-001), (5-10 µg/1x106 cells).
    The control antigen is not suitable for this application.
    Live cell imaging / Immunocytochemistry
    Expression of Zinc-Activated Channel (ZAC) in human PANC-1 cells
    Immunocytochemical staining of human intact living pancreatic adenocarcinoma (PANC-1) cells. Extracellular staining (green) of cells with Anti-Human Zinc-Activated Channel (ZAC) (extracellular) Antibody (#AZC-001), (1:25) followed by goat anti-rabbit-AlexaFluor-488 secondary antibody. DAPI is used as the counterstain (blue).
    References
    1. Davies, P.A. et al. (2003) J. Biol. Chem. 278, 712.
    2. Houtani, T. et al. (2005) Biochem. Biophys. Res. Comm. 335, 277.
    Scientific background

    Over forty homologous proteins belong to the superfamily of ligand-gated ion channels. These members are broken down into different subfamilies, for example: acetylcholine, serotonin and glycine receptors.

    A new member has been added to this superfamily – ZAC (Zinc-Activated Channel) which is expressed in human and dog but not in rodents1. Like all other ligand-gated ion channels, ZAC has four transmembrane domains and extracellular N- and C-termini. As its name states, the channel is activated by high concentrations of zinc.

    ZAC mRNA was found to be expressed in placenta, spinal cord, stomach, and fetal brain1. In another study, ZAC was identified using a computer-assisted program and was detected in similar areas (including pancreas and kidney), but also displayed minor discrepancies2.

    Being that the channel is expressed in human and dog, deciphering its cellular/physiological functions will be challenging.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 01/11/2018

    Anti-Human Zinc-Activated Channel (ZAC) (extracellular) Antibody (#AZC-001) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunocytochemistry, and indirect flow cytometry applications. The antibody recognizes an extracellular epitope, and is thus ideal for detecting ZAC in living cells. It has been designed to recognize ZAC from human samples only.

    For research purposes only, not for human use
    Citations
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