Anti-Human ZACN (extracellular) Antibody

Zinc-activated ligand-gated ion channel, Ligand-gated ion channel zinc-activated 1, Ligand-gated ion-channel receptor L2, ZAC, LGICZ1
    Cat #: AZC-001
    Alternative Name Zinc-activated ligand-gated ion channel, Ligand-gated ion channel zinc-activated 1, Ligand-gated ion-channel receptor L2, ZAC, LGICZ1
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h
      • Peptide CNFELLHFPRDHSN, corresponding to amino acid residues 157-170 of human ZACN (Accession Q401N2). Extracellular, N-terminus.
        Anti-Human ZACN (extracellular) Antibody
    Accession (Uniprot) Number Q401N2
    Gene ID 353174
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Human.
    RRID AB_11219142.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ifc, lci, wb
    May also work in: ih*, ip*
      • Anti-Human ZACN (extracellular) Antibody
        Western blot analysis of PANC-1 (lanes 1 and 3) and Jurkat (lanes 2 and 4) cell lysates:
        1,2. Anti-Human ZACN (extracellular) Antibody (#AZC-001), (1:200).
        3,4. Anti-Human ZACN (extracellular) Antibody, preincubated with the negative control antigen.
      • Anti-Human ZACN (extracellular) Antibody
        Cell surface detection of ZAC in Jurkat living cells:
        ___ Unstained cells.
        ___ Cells + Anti-Human ZACN (extracellular) Antibody (#AZC-001), (5-10 µg/1x106 cells).
        The negative control antigen is not suitable for this application.
      • Anti-Human ZACN (extracellular) Antibody
        Expression of Zinc-activated channel in human PANC-1 cells
        Cell surface detection of Zinc-activated channel in human intact living pancreatic adenocarcinoma (PANC-1) cells. Extracellular staining (green) of cells with Anti-Human ZACN (extracellular) Antibody (#AZC-001), (1:25) followed by goat anti-rabbit-AlexaFluor-488 secondary antibody. DAPI is used as the counterstain (blue).
    References
    • 1. Davies, P.A. et al. (2003) J. Biol. Chem. 278, 712.
    • 2. Houtani, T. et al. (2005) Biochem. Biophys. Res. Comm. 335, 277.
      • Over forty homologous proteins belong to the superfamily of ligand-gated ion channels. These members are divided into subfamilies, for example: acetylcholine, serotonin and glycine receptors.

        A new member has been added to this superfamily – ZAC (Zinc-activated channel) which is expressed in humans and dogs but not in rodents1. Like all other ligand-gated ion channels, ZAC has four transmembrane domains and extracellular N- and C-termini. As its name states, the channel is activated by high concentrations of zinc.

        ZAC mRNA was found to be expressed in the placenta, spinal cord, stomach, and fetal brain1. In another study, ZAC was identified using a computer-assisted program and was detected in similar areas (including pancreas and kidney), but also displayed minor discrepancies2.

        Being that the channel is expressed in human and dog, deciphering its cellular/physiological functions will be challenging.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IF- Immunofluorescence, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 10/11/2019

    Anti-Human ZACN (extracellular) Antibody (#AZC-001) is a highly specific antibody directed against an epitope of the human Zinc-activated ligand-gated ion channel. The antibody can be used in western blot, immunocytochemistry, and indirect flow cytometry applications. The antibody recognizes an extracellular epitope, and is thus ideal for detecting ZAC in living cells. It has been designed to recognize ZAC from human samples only.

    For research purposes only, not for human use
    Citations
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