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Anti-ZIP1 (SLC39A1) (extracellular) Antibody

Zinc transporter ZIP1, Zinc-iron-regulated transporter-like, Zrt- and Irt-like protein 1, ZIRTL

Cat #: AZT-001
Alternative Name Zinc transporter ZIP1, Zinc-iron-regulated transporter-like, Zrt- and Irt-like protein 1, ZIRTL
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)GEPELLVWRPEAVASE, corresponding to amino acid residues 5-20 of human ZIP1 (Accession Q9NY26). Extracellular, N-terminus.
Accession (Uniprot) Number Q9NY26
Gene ID 27173
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, rat - 15/16 amino acid residues identical.
RRID AB_2756808.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ifc, ih, lci, wb
May also work in: ic*, ip*
Western blot
  • Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
    Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
    1,2. Anti-ZIP1 (SLC39A1) (extracellular) Antibody (#AZT-001), (1:200).
    3,4. Anti-ZIP1 (SLC39A1) (extracellular) Antibody, preincubated with ZIP1/SLC39A1 (extracellular) Blocking Peptide (#BLP-ZT001).
  • Western blot analysis of human U-87 MG glioblastoma cell lysate (lanes 1 and 3) and human MDA-231 breast adenocarcinoma cell lysate (lanes 2 and 4):
    Western blot analysis of human U-87 MG glioblastoma cell lysate (lanes 1 and 3) and human MDA-231 breast adenocarcinoma cell lysate (lanes 2 and 4):
    1,2. Anti-ZIP1 (SLC39A1) (extracellular) Antibody (#AZT-001), (1:200).
    3,4. Anti-ZIP1 (SLC39A1) (extracellular) Antibody, preincubated with ZIP1/SLC39A1 (extracellular) Blocking Peptide (#BLP-ZT001).
Immunohistochemistry
  • Expression of ZIP1 in rat hippocampus
    Expression of ZIP1 in rat hippocampus
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-ZIP1 (SLC39A1) (extracellular) Antibody (#AZT-001), (1:400), followed by anti-rabbit-Alexa-488 antibody. ZIP1 is expressed (green) in the mossy fiber (MF) terminal field (arrows) of the CA3 hippocampal region. Nuclei are labeled with DAPI (blue).
Indirect flow cytometry
  • Cell surface detection of ZIP1 (SLC39A1) by indirect flow cytometry in live intact human K562 chronic myelogenous leukemia cells:
    Cell surface detection of ZIP1 (SLC39A1) by indirect flow cytometry in live intact human K562 chronic myelogenous leukemia cells:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells +  Anti-ZIP1 (SLC39A1) (extracellular) Antibody (#AZT-001), (5μg) + goat-anti-rabbit-FITC.
References
  1. Jeong, J. and Eide, D.J. (2013) Mol. Aspects Med. 34, 612.
  2. Franklin, R.B. and Costello, L.C. (2007) Arch. Biochem. Biophys. 463, 211.
  3. Jiang, Y. et al. (2014) PLoS One 9, e116043.
Scientific background

Zinc uptake transporter, ZIP1, belongs to the SLC39 transporter family. This transport has the ability to increase zinc concentration when cellular zinc level is low, by active zinc transport into the cytoplasm from extracellular space of the cell or the intracellular storage compartment. Thus, zinc transport plays a crucial role in regulating cellular zinc homeostasis1-3.

Regulation and maintenance of proper concentration and distribution of cellular zinc are essential to the function, metabolism, growth, proliferation and survival of cells. Zinc deficiency can cause defects in the immune system, including abnormalities in T-cells, natural killer cells and monocytes, and reduction in antibody formation1,2.

In the human genome, 20 ZIP transporters have been identified.  ZIP1 is encoded by the SLC39A1 gene3.

ZIP proteins are highly conserved. Members have eight predicted transmembrane (TM) domains with extracellular N- and C-termini. There is a long loop region harboring a histidine-rich domain between TM3 and TM4. Zinc uptake by ZIP1 is energy independent2.

ZIP1 is ubiquitously expressed in human tissues including blood, brain, gill, heart, intestine, liver, muscle, skin, spleen and kidney3.

Accumulation of zinc in prostate cells by ZIP1 inhibits their growth and proliferation. Therefore, ZIP1 is associated as a tumor suppressor gene in prostate cancer2.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 02/08/2022

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the human Zinc transporter ZIP1. Anti-ZIP1 (SLC39A1) (extracellular) Antibody (#AZT-001) can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the protein in living cells. It has been designed to recognize ZIP1 from mouse, rat and human samples.

For research purposes only, not for human use
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