ZIP1/SLC39A1 (extracellular) Blocking Peptide (#BLP-ZT001) is the original antigen used for immunization during Anti-ZIP1 (SLC39A1) (extracellular) Antibody (#AZT-001) generation. The blocking peptide binds and ‘blocks’ Anti-ZIP1/SLC39A1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
- Western blot analysis of human U-87 MG glioblastoma cell lysate (lanes 1 and 3) and human MDA-231 breast adenocarcinoma cell lysate (lanes 2 and 4):
- Expression of ZIP1 in rat hippocampusImmunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-ZIP1 (SLC39A1) (extracellular) Antibody (#AZT-001), (1:400), followed by anti-rabbit-Alexa-488 antibody. ZIP1 is expressed (green) in the mossy fiber (MF) terminal field (arrows) of the CA3 hippocampal region. Nuclei are labeled with DAPI (blue).
- (C)GEPELLVWRPEAVASE, corresponding to amino acid residues 5-20 of human ZIP1 (Accession Q9NY26).