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Anti-ZIP14 (SLC39A14) Antibody

Zinc Transporter ZIP14, Metal Cation Symporter ZIP14, Zrt- and Irt-like protein 14, Solute Carrier Family 39 Member 14

Cat #: AZT-024
Alternative Name Zinc Transporter ZIP14, Metal Cation Symporter ZIP14, Zrt- and Irt-like protein 14, Solute Carrier Family 39 Member 14
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)KDQEEGVTEKLQNGD, corresponding to amino acid residues 264 - 278 of mouse SLC39A14 (Accession Q75N73). Intracellular, 2nd loop.
Accession (Uniprot) Number Q75N73
Gene ID 213053
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat - identical, Human - 14 out of 15 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preadsorption Control 1 µg peptide per 1 µg antibody
Standard quality control of each lot Western blot analysis.
Applications: ih, wb
May also work in: ic*, ip*
Western blot
  • Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
    Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
    1-2. Anti-ZIP14 (SLC39A14) Antibody (#AZT-024), (1:200).
    3-4. Anti-ZIP14 (SLC39A14) Antibody, preincubated with ZIP14 (SLC39A14) Blocking Peptide (BLP-ZT024).
  • Western blot analysis of mouse heart membranes (lanes 1 and 3) and rat heart membranes (lanes 2 and 4):
    Western blot analysis of mouse heart membranes (lanes 1 and 3) and rat heart membranes (lanes 2 and 4):
    1-2. Anti-ZIP14 (SLC39A14) Antibody (#AZT-024), (1:200).
    3-4. Anti-ZIP14 (SLC39A14) Antibody, preincubated with ZIP14 (SLC39A14) Blocking Peptide (BLP-ZT024).
  • Western blot analysis of human LNCaP prostate carcinoma cell line lysates (lanes 1 and 3) and human THP-1 monocytic leukemia cell line lysates (lanes 2 and 4):
    Western blot analysis of human LNCaP prostate carcinoma cell line lysates (lanes 1 and 3) and human THP-1 monocytic leukemia cell line lysates (lanes 2 and 4):
    1-2. Anti-ZIP14 (SLC39A14) Antibody (#AZT-024), (1:500).
    3-4. Anti-ZIP14 (SLC39A14) Antibody, preincubated with ZIP14 (SLC39A14) Blocking Peptide (BLP-ZT024).
Immunohistochemistry
  • Expression of ZIP14 in mouse striatum.
    Expression of ZIP14 in mouse striatum.
    Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-ZIP14 (SLC39A14) Antibody (#AZT-024), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. ZIP14 immunoreactivity (green) appears in neuron profiles (arrows). B. Pre-incubation of the antibody with ZIP14 (SLC39A14) Blocking Peptide (BLP-ZT024), suppressed staining. Cell nuclei are stained with DAPI (blue).
  • Expression of ZIP14 in mouse cerebellum.
    Expression of ZIP14 in mouse cerebellum.
    Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-ZIP14 (SLC39A14) Antibody (#AZT-024), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. ZIP14 immunoreactivity (green) appears in neuron profiles (arrows) and diffusely in the molecular layer (MOL). B. Pre-incubation of the antibody with ZIP14 (SLC39A14) Blocking Peptide (BLP-ZT024), suppressed staining. Cell nuclei are stained with DAPI (blue).
References
  1. Nelson. N, et al (1999), EMBO Journal. 18, 4371.
  2. Eide.D, et al (2004), Pflugers Arch. 10, 1074.
  3. Aydemir.T, et al (2018), Journal of Nutrition. 174–184.
  4. Winslow. J, et al (2020), Department of Nutritional Sciences. 21, 3304.
Scientific background

Metal ions are essential elements that participate in many different biological processes and therefore their homeostasis is crucial for life. These ions are utilized in oxygen transfer, catalysis, macromolecule stability, gene expression and cell signaling. The homeostasis of metal ions in cells dictated using a specific set of transporters responsible for maintaining their appropriate concentration1.  

SLC39A14 (ZIP14) is a member of the the Zrt – and – Irt like family (ZIP) a large family of metal ion transporters that can be found in organisms across all kingdoms of life. ZIP14 function as a transporter of zinc, iron, manganese and cadmium across cellular membranes2.

According to ZIP14 predicted structure it consists of 8 transmembrane domains (TMDs), extracellular N-terminus and C-terminus and several histidine reach repeats. Structural studies of ZIP14 using SDS-PAGE analysis under nonreducing conditions was show that ZIP14 undergoes trimerization3.

Human ZIP14 mRNA was detected in the liver, pancreases, thyroid and in the heart. In addition, mouse mRNA was also detected in the kidney, white adipose tissue (WAT), skeletal muscle and spleen. Mutations in gene encoding ZIP14 leads to manganese toxicity and early-onset dystonia in humans, highlighting ZIP14 important role in manganese homeostasis4.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 01/08/2021

Anti-ZIP14 (SLC39A14) Antibody (#AZT-024) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize ZIP14 from rat, mouse and human samples.

For research purposes only, not for human use
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