Aquaporin 2 Blocking Peptide (#BLP-QP002) is the original antigen used for immunization during Anti-Aquaporin 2 Antibody (#AQP-002) generation. The blocking peptide binds and ‘blocks’ Anti-Aquaporin 2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat kidney membranes:1. Anti-Aquaporin 2 Antibody (#AQP-002), (1:200).
2. Anti-Aquaporin 2 Antibody, preincubated with a blocking peptide.
- Expression of AQP2 in rat kidneyImmunohistochemical staining of rat kidney paraffin embedded section showing the inner medulla using Anti-Aquaporin 2 Antibody (#AQP-002), (1:100). Intense stain (brown color) is present in collecting ducts but not in thin segments of the loop of Henle. Hematoxilin is used as the counterstain.
- Mouse paraffin-embedded kidney sections (1:200) (Kim, J.I. et al. (2013) Am. J. Physiol. 304, F1283.).
- (C)RQSVELHSPQSLPRGSKA, corresponding to amino acid residues 254-271 of rat AQP2 (Accession P34080).