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Anti-Aquaporin 2 Antibody

AQP2, AQP-CD, WCH-CD

Cat #: AQP-002
Alternative Name AQP2, AQP-CD, WCH-CD
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)RQSVELHSPQSLPRGSKA, corresponding to amino acid residues 254-271 of rat AQP2 (Accession number P34080). Intracellular, C-terminus.
Accession (Uniprot) Number P34080
Gene ID 25386
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - identical; human, sheep - 17/18 amino acid residues identical.
RRID AB_2039728.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.6 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, ih, wb
May also work in: ifc*, ip*
Western blot
  • Western blot analysis of rat kidney membranes:
    Western blot analysis of rat kidney membranes:
    1. Anti-Aquaporin 2 Antibody (#AQP-002), (1:200).
    2. Anti-Aquaporin 2 Antibody, preincubated with a blocking peptide.
Immunohistochemistry
  • Expression of AQP2 in rat kidney
    Expression of AQP2 in rat kidney
    Immunohistochemical staining of rat kidney paraffin embedded section showing the inner medulla using Anti-Aquaporin 2 Antibody (#AQP-002), (1:100). Intense stain (brown color) is present in collecting ducts but not in thin segments of the loop of Henle. Hematoxilin is used as the counterstain.
  • Mouse paraffin-embedded kidney sections (1:200) (Kim, J.I. et al. (2013) Am. J. Physiol. 304, F1283.).
References
  1. King, L.S. et al. (2004) Nat. Rev. Mol. Cell Biol. 5, 687.
  2. Deen, P.M. et al. (1994) Science 264, 92.
  3. Nielsen, S. et al. (2002) Physiol. Rev. 82, 205.
  4. Robben, J.H. et al. (2006) Am. J. Physiol. Renal Physiol. 291, F257.
Scientific background

Aquaporin 2 (AQP2) belongs to a family of membrane proteins that allow passage of water and certain other solutes through biological membranes. The family is composed of 13 members (AQP0 to AQP12). Little is known about the function of the two newest members, AQP11 and AQP12.

The aquaporins can be divided into two functional groups based on their permability characteristics: the aquaporins that are only permeated by water and the aquaglyceroporins that are permeated by water and other small solutes such as glycerol. AQP2 together with AQP1AQP4 and AQP5 belongs to the first group.1

The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit, contains four pores.1

AQP2 expression is largely confined to the kidney, particularly in the renal collecting duct where it performs a key role in water absorption and urine concentration. In fact, mutations in the AQP2 gene produce hereditary nephrogenic diabetes insipidus, a disorder that results in the excretion of large volumes of urine.2

Under normal conditions, water homeostasis in the kidney is regulated through the anti-diuretic hormone vasopressin. Vasopressin is secreted from the pituitary gland and transported to the kidney through the blood where it binds to its receptor that is mainly expressed in cells of the collecting duct. The activated vasopressin receptor induces an increase in intracellular cAMP and subsequent PKA activation, which phosphorylates AQP2. This phosphorylation causes the translocation of AQP2 channels from intracellular vesicles to the cell membrane where it markedly increases water permeability.1,3,4

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Image & Title: Anti-Aquaporin 2 AntibodyExpression of AQP2 in rat kidney sections before and after gentamicin treatment.Immunohistochemical staining of rat kidney sections using Anti-Aquaporin 2 Antibody (#AQP-002) after GM treatment. Kidney sections from rats after 2 days of treatment with vehicle (control; A, D, and G) or GM (B, E, and H) or 8 days of treatment with GM (C, F, I, and J). Representative images of the cortex (A–C), outer medulla (D–F), and inner medulla (G–J) are shown. The box in I indicates the region of high magnification in J. Brown staining indicates the presence of AQP2.Adapted from Abdeen, A. et al. (2014) with permission of the American Physiological Society.
Last update: 21/12/2020

Anti-Aquaporin 2 Antibody (#AQP-002) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize the AQP2 channel from rat, mouse, and human samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Western blot citations
  1. Rat kidney lysate.
    Chung, S. et al. (2019) Front. Physiol. 10, 271.
  2. Rat RCCD1 and mouse mpkCCDC14 cell lysates.
    Mazzocchi, M. et al. (2019) J. Physiol. Biochem. 76, 37.
  3. Mouse cortical collecting duct mpkCCDC14 cells.
    Al-Bataineh, M.M. et al. (2016) Am. J. Physiol. 311, F890.
  4. Rat kidney lysate (1:1500).
    Mamenko, M. et al. (2016) J. Am. Soc. Nephrol. 27, 2035.
Immunohistochemistry citations
  1. Rat kidney sections.
    Chung, S. et al. (2019) Front. Physiol. 10, 271.
  2. Mouse kidney sections.
    Kong, M.J. et al. (2019) Redox Biol. 20, 38.
  3. Mouse kidney sections (1:2000).
    Sun, X. et al. (2015) Am. J. Physiol. 309, F120.
  4. Rat kidney sections (1:100).
    Abdeen, A. et al. (2014) Am. J. Physiol. 307, F1227.
  5. Mouse paraffin-embedded kidney sections (1:200).
    Kim, J.I. et al. (2013) Am. J. Physiol. 304, F1283.
Immunocytochemistry citations
  1. Rat RCCD1 and mouse mpkCCDC14 cells (1:100).
    Mazzocchi, M. et al. (2019) J. Physiol. Biochem. 76, 37.
More product citations
  1. Kim, J. et al. (2010) Am. J. Physiol. 298, F1118.
  2. Satake, M. et al. (2010) Biol. Pharm. Bull. 33, 1965.
  3. Bae, E.H. et al. (2009) Nephrol. Dial. Transplant. 24, 2692.
  4. Kim, J. et al. (2009) Am. J. Physiol. 296, F622.
  5. Allory, Y. et al. (2008) Kidney Int. 73, 751.
  6. Bae, E.H. et al. (2008) Am. J. Physiol. Renal Physiol. 294, F272.
  7. Lodewyck, D. et al. (2007) Arch Otolaryngol Head Neck Surg. 133, 557.
  8. Kim, D. et al. (2007) J. Am. Soc. Nephrol. 18, 1458.
  9. Ma, S.K. et al. (2007) Kidney Blood Press. Res. 30, 8.
  10. Watson, K.J. et al. (2007) Chem. Senses. 32, 411.
  11. Sun, P. et al. (2006) Am. J. Physiol. 291, F1192.
  12. Rahmoune, H. et al. (2005) Diabetes 54, 3427.
  13. Behr, R. et al. (2004) J. Biol. Chem. 279, 41936.
  14. Kim S.W. et al. (2004) J. Am. Soc. Nephrol. 15, 2998.
  15. Lin, D.H. et al. (2004) Am. J. Physiol. 286, F881.
  16. Wei, Y. et al. (2004) J. Gen. Physiol. 124, 719.
  17. Hirano, K. et al. (2003) Am. J. Pathol. 163, 111.
  18. Rubera, I. et al. (2003) J. Clin. Invest. 112, 554.
  19. Yeum, C.H. et al. (2003) Scand. J. Urol. Nephrol. 37, 99.
  20. Gallardo, P. et al. (2001) Am. J. Physiol. Gastrointest. Liver Physiol. 281, G856.
  21. Kim, S.W. et al. (2001) J. Am. Soc. Nephrol. 12, 875.
  22. Mittal, A. et al. (2000) Life Sci., 66, 1471.
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