Aquaporin 4/AQP4 (249-323) Blocking Peptide (#BLP-QP004) is the original antigen used for immunization during Anti-Aquaporin 4 (AQP4) (249-323) Antibody (#AQP-004) generation. The blocking peptide binds and ‘blocks’ Anti-Aquaporin 4/AQP4 (249-323) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain membranes:1. Anti-Aquaporin 4 (AQP4) (249-323) Antibody (#AQP-004), (1:1000).
2. Anti-Aquaporin 4 (AQP4) (249-323) Antibody, preincubated with the control fusion protein (BLP-QP004).
- GST fusion protein with the sequence EYVFCPDVELKRRLKEAFSKAAQQTKGSYMEVEDNRSQVETEDLILKPGVVHVIDIDRGDEKKGKDSSGEVLSSV, corresponding to amino acid residues 249-323 of rat AQP4 (Accession P47863).