ASCT2/SLC1A5 Blocking Peptide (#BLP-NT082) is the original antigen used for immunization during Anti-ASCT2 (SLC1A5) Antibody (#ANT-082) generation. The blocking peptide binds and ‘blocks’ Anti-ASCT2/SLC1A5 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
- Expression of ASCT2 in mouse hippocampusImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-ASCT2 (SLC1A5) Antibody (#ANT-082), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. ASCT2 staining appears in a layer (arrows) adjacent to the dentate granule layer (GL). Cell nuclei are stained with DAPI (blue).
- (C)KMPSSEPELIQVKNE, corresponding to amino acid residues 502-516 of mouse SLC1A5 (Accession P51912).