ASIC3 Blocking Peptide (#BLP-SC018) is the original antigen used for immunization during Anti-ASIC3 Antibody (#ASC-018) generation. The blocking peptide binds and ‘blocks’ Anti-ASIC3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat DRG lysate:
- Multiplex staining of ASIC1 and ASIC3 in rat DRGImmunohistochemical staining of perfusion-fixed frozen rat dorsal root ganglia (DRG) sections using Guinea pig Anti-ASIC1 Antibody (#ASC-014-GP), (1:400) and rabbit Anti-ASIC3 Antibody (#ASC-018), (1:400). A. ASIC3 staining. B. ASIC1 staining in same section (green). C. Merge of the two images shows some co-localization (arrow). Cell nuclei were stained with DAPI (blue).
- Rat trigeminal ganglia (1:400) (Yan, J. et al. (2013) Headache 53, 1250.).
- Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
- Western blot analysis of rat dorsal root ganglion (DRG) lysate:
- Expression of ASIC3 in mouse dorsal raphe nucleusImmunohistochemical staining of perfusion-fixed frozen mouse brain sections using Guinea pig Anti-ASIC3 Antibody (#ASC-018-GP), (1:200), followed by goat-anti-guinea pig-AlexaFluor-488 secondary antibody. ASIC3 staining (green) appears in the soma of dorsal raphe nucleus (DRN) neurons (arrows). Nuclei are stained with DAPI (blue).
- KPRSGLEEAQRRQAS(C), corresponding to amino acid residues 2-16 of rat ASIC3 (Accession O35240).