Bombesin Receptor 1/NMBR Blocking Peptide (#BLP-BR004) is the original antigen used for immunization during Anti-Bombesin Receptor 1 (NMBR) Antibody (#ABR-004) generation. The blocking peptide binds and ‘blocks’ Anti-Bombesin Receptor 1/NMBR primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
- Western blot analysis of human lung carcinoma NCI-H526 (lanes 1 and 3) and human prostate carcinoma PC-3 (lanes 2 and 4) cell line lysates:
- Expression of Bombesin receptor 1 in rat hypothalamusImmunohistochemical staining of frozen rat hypothalamus sections using Anti-Bombesin Receptor 1 (NMBR) Antibody (#ABR-004), (1:200). A. Staining (red) appears in the neuropil near the ventricle (asterisk) and in neurons (triangles). Calbindin D28k staining (green) appears in neurons. C. Merge of (A) and (B) shows co-expression of BB1R and Calbindin in a few neurons (vertical arrow). DAPI is used as the counterstain.
- (C)KSAHNLPGEYNEHTKK, corresponding to amino acid residues 241-256 of human BB1R (Accession P28336).