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Certificate of Analysis

C5aR2 (extracellular) Blocking Peptide

Overview

Cat #: BLP-AR034
Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized Powder
Applications: bl

Application key:

BL- Blocking
For research purposes only, not for human use

Properties

Properties

Sequence
  • Peptide (C)RRLLQEHYPPRLV(S)G, corresponding to amino acid residues 179 - 193 of mouse C5aR2 (Accession Q8BW93).
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 2 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.

Applications

Applications

Blocking
  • Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):
    Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):
    1-2. Anti-C5aR2 (extracellular) Antibody (#AAR-034), (1:400).
    3-4. Anti-C5aR2 (extracellular) Antibody, preincubated with C5aR2 (extracellular) Blocking Peptide (BLP-AR034).
  • Western blot analysis of mouse J774 macrophage cell line lysate (lanes 1 and 3) and mouse M1 myeloid leukemia cell line lysate (lanes 2 and 4):
    Western blot analysis of mouse J774 macrophage cell line lysate (lanes 1 and 3) and mouse M1 myeloid leukemia cell line lysate (lanes 2 and 4):
    1-2. Anti-C5aR2 (extracellular) Antibody (#AAR-034), (1:400).
    3-4. Anti-C5aR2 (extracellular) Antibody, preincubated with C5aR2 (extracellular) Blocking Peptide (BLP-AR034).
Last update: 11/04/2021
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