Overview
Type: Synthetic peptide
Form: Lyophilized powder
CACNG8 Blocking Peptide (#BLP-CC125) is the original antigen used for immunization during Anti-CACNG8 Antibody (#ACC-125) generation. The blocking peptide binds and ‘blocks’ Anti-CACNG8 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat testis (lanes 1 and 4), rat brain (lanes 2 and 5) and mouse brain (lanes 3 and 6) lysates:1-3. Anti-CACNG8 Antibody (#ACC-125), (1:400).
4-6. Anti-CACNG8 Antibody, preincubated with CACNG8 Blocking Peptide (#BLP-CC125).
Expression of TARP γ8 (CACNG8) in rat hippocampusImmunohistochemical staining of rat hippocampus using Anti-CACNG8 Antibody (#ACC-125). A. TARP γ8 staining (green) appears in neuronal soma (horizontal arrows) and in the neuronal processes (diagonal arrows). B. Nuclear staining using DAPI as the counterstain (blue). C. Merged image of A and B.
Properties
Sequence
- (C)ESLKRWNEERGLW(S)EK, corresponding to amino acid residues 2-17 of rat CACNG8 (Accession Q8VHW5).
Accession (Uniprot) Number Q8VHW5
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
