CALHM1 Blocking Peptide (#BLP-CC101) is the original antigen used for immunization during Anti-CALHM1 Antibody (#ACC-101) generation. The blocking peptide binds and ‘blocks’ Anti-CALHM1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain (lanes 1 and 5), mouse brain (lanes 2 and 6), human U-87 MG brain glioblastoma cell line (lanes 3 and 7) and rat C6 brain glioma cell line (lanes 4 and 8) lysates:
- Expression of CALHM1 in rat hippocampusImmunohistochemical staining of rat hippocampus using Anti-CALHM1 Antibody (#ACC-101). A. Staining of CALHM1 appears only in the hippocampal CA1 pyramidal layer (green). B. Nuclear staining using DAPI as the counterstain (blue). C. Merge of A and B.
- (C)EAMNHDLELGHTHG, corresponding to amino acid residues 252-265 of human CALHM1 (Accession Q8IU99).