Overview
Type: Synthetic peptide
Form: Lyophilized powder
CD39 (extracellular) Blocking Peptide (#BLP-NT065) is the original antigen used for immunization during Anti-CD39 (extracellular) Antibody (#ANT-065) generation. The blocking peptide binds and ‘blocks’ Anti-CD39 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only, not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain membranes:1. Anti-CD39 (extracellular) Antibody (#ANT-065), (1:200).
2. Anti-CD39 (extracellular) Antibody, preincubated with CD39 (extracellular) Blocking Peptide (#BLP-NT065).
Western blot analysis of human Jurkat T-cell leukemia cell line (lanes 1 and 3) and human THP-1 monocytic leukemia cell line (lanes 2 and 4) lysates:1,2. Anti-CD39 (extracellular) Antibody (#ANT-065), (1:400).
3,4. Anti-CD39 (extracellular) Antibody, preincubated with CD39 (extracellular) Blocking Peptide (#BLP-NT065).
Western blot analysis of mouse BV-2 microglia cell line (lanes 1 and 3) and human HMC3 microglia cell line (lanes 2 and 4) lysates:1,2. Anti-CD39 (extracellular) Antibody (#ANT-065), (1:200).
3,4. Anti-CD39 (extracellular) Antibody, preincubated with CD39 (extracellular) Blocking Peptide (#BLP-NT065).
Properties
Sequence
- (C)KTSYAGVKEKYLSE, corresponding to amino acid residues 398-411 of human CD39 (Accession P49961).
Accession (Uniprot) Number P49961
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
