CD56/NCAM1 (extracellular) Blocking Peptide (#BLP-NR041) is the original antigen used for immunization during Anti-CD56/NCAM1 (extracellular) Antibody (#ANR-041) generation. The blocking peptide binds and ‘blocks’ Anti-CD56/NCAM1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse brain (lanes 1 and 6), rat cerebellum (lanes 2 and 7), rat eye (lanes 3 and 8), human SH-SY5Y brain neuroblastoma (lanes 4 and 9) and PC12 rat pheochromocytoma (lanes 5 and 10) cells lysates:
- Expression of NCAM1 in rat olfactory bulbImmunohistochemical staining of rat olfactory bulb frozen section using Anti-CD56/NCAM1 (extracellular) Antibody (#ANR-041), (1:300). A. NCAM1 staining (green) appears in glomeruli (G) and is particularly intense in the outer border (arrows). B. Nuclei are stained using DAPI as the counterstain. C. Merged image of A and B panels.
- (C)DEKHIFSDDSSELTIR, corresponding to amino acid residues 261-276 of rat NCAM-1 (Accession P13596).