Overview
Type: Synthetic peptide
Form: Lyophilized powder
CD73 (extracellular) Blocking Peptide (#BLP-NT066) is the original antigen used for immunization during Anti-CD73 (extracellular) Antibody (#ANT-066) generation. The blocking peptide binds and ‘blocks’ Anti-CD73 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):1,2. Anti-CD73 (extracellular) Antibody (#ANT-066), (1:200).
3,4. Anti-CD73 (extracellular) Antibody, preincubated with CD73 (extracellular) Blocking Peptide (#BLP-NT066).
Western blot analysis of human U-87 MG glioblastoma cell line lysate (lanes 1 and 3) and human MDA-MB-231 breast adenocarcinoma cell line lysate (lanes 2 and 4):1,2. Anti-CD73 (extracellular) Antibody (#ANT-066), (1:200).
3,4. Anti-CD73 (extracellular) Antibody, preincubated with CD73 (extracellular) Blocking Peptide (#BLP-NT066).
Expression of CD73 in mouse striatum and hippocampus.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-CD73 (extracellular) Antibody (#ANT-066), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. CD73 staining (green) in mouse striatum is detected in striatal cells (arrows). B. CD73 staining (green) in hippocampal CA1 region is detected in cells of the pyramidal layer (vertical arrow) and their apical dendrites (horizontal arrows). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)KGSTLKKAFEHSVHR, corresponding to amino acid residues 429-433 of mouse CD73 (Accession Q61503).
Accession (Uniprot) Number Q61503
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
