CD73 (extracellular) Blocking Peptide (#BLP-NT066) is the original antigen used for immunization during Anti-CD73 (extracellular) Antibody (#ANT-066) generation. The blocking peptide binds and ‘blocks’ Anti-CD73 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):
- Western blot analysis of human U-87 MG glioblastoma cell line lysate (lanes 1 and 3) and human MDA-MB-231 breast adenocarcinoma cell line lysate (lanes 2 and 4):
- Expression of CD73 in mouse striatum and hippocampus.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-CD73 (extracellular) Antibody (#ANT-066), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. CD73 staining (green) in mouse striatum is detected in striatal cells (arrows). B. CD73 staining (green) in hippocampal CA1 region is detected in cells of the pyramidal layer (vertical arrow) and their apical dendrites (horizontal arrows). Cell nuclei are stained with DAPI (blue).
- (C)KGSTLKKAFEHSVHR, corresponding to amino acid residues 429-433 of mouse CD73 (Accession Q61503).