CDNF Blocking Peptide (#BLP-NT027) is the original antigen used for immunization during Anti-CDNF Antibody (#ANT-027) generation. The blocking peptide binds and ‘blocks’ Anti-CDNF primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of 100 ng human recombinant CDNF (lanes 1 and 3) and 100 ng human recombinant MANF (lanes 2 and 4):
- Western blot analysis of mouse brain lysate:
- Expression of CDNF in mouse brainImmunohistochemical staining of immersion-fixed, free floating mouse brain frozen sections using rabbit Anti-CDNF Antibody (#ANT-027), (1:300). A. CDNF staining (green) is detected in several cells in the vicinity of the substantia nigra pars compacta. B. Tyrosine hydroxylase staining (red) appears in the substantia nigra. C. Merge of the two images demonstrates dopaminergic (TH-positive) neurons also expressing CDNF (arrows). Cell nuclei are stained with DAPI (blue).
- (C)EKTLDLASVDLRK, corresponding to amino acid residues 129-141 of human CDNF (Accession Q49AH0).