CHRM5 (extracellular) Blocking Peptide (#BLP-MR005) is the original antigen used for immunization during Anti-CHRM5 (extracellular) Antibody (#AMR-005) generation. The blocking peptide binds and ‘blocks’ Anti-CHRM5 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat lung (lanes 1 and 5) and brain (lanes 2 and 6) membranes, human A-875 melanoma (lanes 3 and 7) and human MALM-3M malignant melanoma (lanes 4 and 8) cell lysates:
- (C)HNATTVNGTPVNHQPLER, corresponding to amino acid residues 7-24 of human M5 muscarinic receptor (Accession P08912).