CNGA3 Blocking Peptide (#BLP-PC060) is the original antigen used for immunization during Anti-CNGA3 Antibody (#APC-060) generation. The blocking peptide binds and ‘blocks’ Anti-CNGA3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain membranes:
- Expression of CNG3 in rat brainImmunohistochemical staining of frozen rat hippocampal dentate gyrus (DG) sections using Anti-CNGA3 Antibody (#APC-060). Neurons were found in the hilus of the DG with dendritic processes extending across the granule cell layer (arrows) both in the dorsal blade (A) and ventral blade (B). Diaminobenzidine color product is blue-black (arrows). DAPI is used as the counterstain (silver).
- (C)GHGFSPDRENSEDASKAD, corresponding to amino acid residues 594-611 of rat CNGA3 (Accession Q9QWN7).