Connexin-26/GJB2 Blocking Peptide (#BLP-CC212) is the original antigen used for immunization during Anti-Connexin-26 (GJB2) Antibody (#ACC-212) generation. The blocking peptide binds and ‘blocks’ Anti-Connexin-26/GJB2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) liver membranes:
- Human fetal cochlear sections (Locher, H. et al. (2015) Dev. Neurobiol. 75, 1219.).
- (C)HEKKRKFMKGEIK, corresponding to amino acid residues 100-112 of rat Connexin-26 (Accession P21994).