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Contulakin-G

A Neurotensin Receptor Ligand
Cat #: GPC-100
Lyophilized Powder yes
  • Bioassay Tested
  • Origin Synthetic peptide
    MW: 1703 Da.
    Purity: >98% (HPLC)
    Effective concentration 100 nM - 1 µM.
    Sequence ZSEEGGSNATKKPYIL.
    Modifications Z = Pyrrolidone carboxylic acid.
    Structure
    Molecular formula C74H117N19O27.
    CAS No.: 229180-41-0.
    Activity Contulakin-G acts as an agonist of neurotensin receptors. It binds to human neurotensin type 1 receptor (NTSR1), rat neurotensin types 1 and 2 receptors (NTSR1/NTSR2) and mouse neurotensin type 3 receptor (SORT1)1.
    References-Activity
    1. Craig A.G. et al. (1999) J. Biol. Chem. 274, 13752.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to two weeks at 4°C or three months at -20°C.
    Our bioassay
    • Alomone Labs Contulakin-G increases intracellular Ca2+ concentration in HT-29 cells.
      Alomone Labs Contulakin-G increases intracellular Ca2+ concentration in HT-29 cells.
      Cells were loaded with Fluo-3 AM. Changes in intracellular Ca2+ were detected via changes in Fluo-3 emission following Contulakin-G (#GPC-100) application. A. Normalized fluorescence before and after application (at 20 seconds, see arrow) of 0-3.3 μM Contulakin-G (as indicated). B. Normalized fluorescence plotted against Contulakin-G concentrations (n = 2, standard deviation indicated by error bars).
    References - Scientific background
    1. Craig A.G. et al. (1999) J. Biol. Chem. 274, 13752.
    2. Craig, A.G. et al. (2001) Toxicon 39, 809.
    Scientific background

    Contulakin-G acts as an agonist of neurotensin receptors. It binds to human neurotensin type 1 receptor (NTSR1), rat neurotensin types 1 and 2 receptors (NTSR1/NTSR2) and mouse neurotensin type 3 receptor (SORT1)1.

    Contulakin-G is a peptide initially isolated and purified from the marine snail Conus geographus venom based on its ability to induce sluggishness in mice2.

    O-linked glycosylation at Threonine residue at position 10 appears to be a highly unusual strategy for increasing the efficacy of toxins directed against neurotransmitter receptors1. Although the binding affinity of Contulakin-G for cloned neurotensin receptors in vitro proved to be significantly lower than neurotensin, Contulakin-G was shown to be a neurotensin receptor agonist at physiologically relevant concentrations1,2.

    Target Neurotensin receptors
    Net Peptide Content: 100%
    Last update: 16/08/2020

    Contulakin-G (#GPC-100) is a highly pure, synthetic, and biologically active peptide toxin.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
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