CRF2/CRHR2 (extracellular) Blocking Peptide (#BLP-CR052) is the original antigen used for immunization during Anti-CRF2/CRHR2 (extracellular) Antibody (#ACR-052) generation. The blocking peptide binds and ‘blocks’ Anti-CRF2/CRHR2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse brain membrane (lanes 1 and 5), rat cerebellum (lanes 2 and 6), rat lung (lanes 3 and 7) and rat kidney (lanes 4 and 8) lysates:
- Western blot analysis of human MOLT-4 acute lymphoblastic leukemia (lanes 1 and 5), human CAKI-1 renal cell carcinoma (lanes 2 and 6), human MEG-O1 megakaryoblastic leukemia (lanes 3 and 7) and human COLO-205 colon adenocarcinoma (lanes 4 and 8) cell line lysates:
- (C)EELLLDGWGEPPDPE, corresponding to amino acid residues 20-34 of rat CRHR2 (Accession P47866).