Cat #: BLP-CR057
Type: Synthetic peptide
Form: Lyophilized powder
A Blocking peptide for Anti-CX3CL1 (extracellular) Antibody (#ACR-057). Intended to be used as a negative control.
For research purposes only, not for human use
- Western blot analysis of rat heart membrane (lanes 1 and 5), human pancreatic carcinoma PANC-1 lysate (lanes 2 and 6), mouse heart membrane (lanes 3 and 7) and rat brain synaptosomal fraction (lanes 4 and 8):
- Expression of CX3CL1 in rat frontal cortexImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-CX3CL1 (extracellular) Antibody (#ACR-057), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. CX3CL1 staining (green) in the deep layer of the frontal cortex is detected in cortical neurons (vertical arrows) and their dendrites (horizontal arrows). B. CX3CL1 immunoreactivity (green) in the upper layer of the frontal cortex is observed in cortical neurons (vertical arrows). Cell nuclei are stained with DAPI (blue).
- CGKRAIILETRQHR, corresponding to amino acid residues 58-71 of human CX3CL1 (Accession P78423).
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
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