[D-Ala7]-Angiotensin I/II (1-7)

A-779
A Potent Agonist of AT1R and Antagonist of Angiotensin-(1-7) Mas Receptor
    Cat #: SPA-160
    Alternative Name A-779
  • Lyophilized Powder
  • Bioassay Tested
  • Origin Synthetic peptide
    MW: 873 Da.
    Purity: ≥98% (HPLC)
    Effective concentration 1-200 µM.
    Sequence DRVYIHa.
    Modifications Ala-7 = D-Ala.
      • [D-Ala7]-Angiotensin I/II (1-7)
    Molecular formula C39H60N12O11.
    CAS No.: 159432-28-7.
    Activity An Angiotensin (1-7) Antagonist1.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility Soluble in water. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to four weeks at 4°C or three months at -20°C.
      • [D-Ala7]-Angiotensin I/II (1-7)
        Alomone Labs [D-Ala7]-Angiotensin I/II (1-7) increases the activation of Angiotensin AT1R expressed in CHO-K1-mt Aequorin-Gα16 cells.
        Ca2+ response, as detected by elevation in aequorin derived fluorescence, in response to increased concentration of [D-Ala7]-Angiotensin I/II (1-7) (#SPA-160), compared to 0.04 nM Angiotensin II. 20 µM [D-Ala7]-Angiotensin I/II (1-7) application resulted in ~65% activation of AT1R, equal to Angiotensin II response.
    References - Scientific background
    • 1. Benter, I.F. et al. (2011) J. Cardiovasc. Pharmacol. 57, 559.
    • 2. Santos, R.A. et al. (1994) Brain Res. Bull. 35, 293.
    • 3. Ambühl, P. et al. (1994) Brain Res. Bull. 35, 289.
      • D-Ala7-Angiotensin I/II (1-7) [D-Ala7-Ang-(1–7)] is a potent and selective synthetic Angiotensin (1-7) Mas receptor antagonist. It has the ability to antagonize several actions of Ang-(1-7), including the antidiuretic effect of the peptide. In addition, it antagonizes the effects of the changes in mean arterial pressure produced by Ang-(1-7) microinjection into the dorsomedial and ventrolateral medulla1-3.

        Ang-(1-7) is an alternative peptide, a product of the renin-angiotensin system (RAS), with special importance in modulating cell-to-cell communication in cardiovascular and neuronal tissue and plays a role in lowering blood pressure and can decrease the sympathetic nervous system activity.

        Ang-(1-7) is formed from Ang I by endopeptidases (neprilysin and prolyl oligopeptidase) or by Ang II by carboxypeptidases such as angiotensin-converting enzyme1,2.

    Target AT1, Angiotensin-(1-7) Mas receptors
    Net Peptide Content: 100%
    Last update: 01/07/2019

    [D-Ala7]-Angiotensin I/II (1-7) (#SPA-160) is a highly pure, synthetic, and biologically active peptide.

    For research purposes only, not for human use