Name: [D-Ala7]-Angiotensin I/II (1-7)
Brand: Alomone Labs
SKU: SPA-160

Overview

Cat #: SPA-160

Alternative Name A-779

Lyophilized Powder yes

Origin Synthetic peptide

MW: 873 Da

Purity: ≥98% (HPLC)

Effective concentration 1-200 µM.

Sequence DRVYIHa.

Modifications Ala-7 = D-Ala.

Structure

Molecular formula C₃₉H₆₀N₁₂O₁₁.

CAS No.: 159432-28-7

Activity An Angiotensin (1-7) Antagonist¹.

References-Activity

Santos, R.A. et al. (1994) Brain Res. Bull. 35, 293.

Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Solubility Soluble in water. Centrifuge all product preparations before use (10000 x g 5 min).

Storage of solutions Up to four weeks at 4°C or three months at -20°C.

Our bioassay

Alomone Labs [D-Ala⁷]-Angiotensin I/II (1-7) increases the activation of Angiotensin AT₁R expressed in CHO-K1-mt Aequorin-Gα16 cells.
Ca²⁺ response, as detected by elevation in aequorin derived fluorescence, in response to increased concentration of [D-Ala⁷]-Angiotensin I/II (1-7) (#SPA-160), compared to 0.04 nM Angiotensin II. 20 µM [D-Ala⁷]-Angiotensin I/II (1-7) application resulted in ~65% activation of AT₁R, equal to Angiotensin II response.

References - Scientific background

Benter, I.F. et al. (2011) J. Cardiovasc. Pharmacol. 57, 559.
Santos, R.A. et al. (1994) Brain Res. Bull. 35, 293.
Ambühl, P. et al. (1994) Brain Res. Bull. 35, 289.

Scientific background

D-Ala⁷-Angiotensin I/II (1-7) [D-Ala⁷-Ang-(1–7)] is a potent and selective synthetic Angiotensin (1-7) Mas receptor antagonist. It has the ability to antagonize several actions of Ang-(1-7), including the antidiuretic effect of the peptide. In addition, it antagonizes the effects of the changes in mean arterial pressure produced by Ang-(1-7) microinjection into the dorsomedial and ventrolateral medulla^1-3.

Ang-(1-7) is an alternative peptide, a product of the renin-angiotensin system (RAS), with special importance in modulating cell-to-cell communication in cardiovascular and neuronal tissue and plays a role in lowering blood pressure and can decrease the sympathetic nervous system activity.

Ang-(1-7) is formed from Ang I by endopeptidases (neprilysin and prolyl oligopeptidase) or by Ang II by carboxypeptidases such as angiotensin-converting enzyme^1,2.

Target AT₁, Angiotensin-(1-7) Mas receptors

Peptide Content: 100%

Lyophilized Powder

[D-Ala⁷]-Angiotensin I/II (1-7) (#SPA-160) is a highly pure, synthetic, and biologically active peptide.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 10/04/2023

Applications

Citations

in vivo

Mice (5 mg/kg), intraperitoneally (i.p.).
Souza, L.K. et al. (2016) Biochem. Pharmacol. 112, 50.

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[D-Ala⁷]-Angiotensin I/II (1-7)

Overview

Applications

Citations

Specifications

Scientific Background

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