D4 Dopamine Receptor Blocking Peptide (#BLP-DR004) is the original antigen used for immunization during Anti-D4 Dopamine Receptor Antibody (#ADR-004) generation. The blocking peptide binds and ‘blocks’ Anti-D4 Dopamine Receptor primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:1,2. Anti-D4 Dopamine Receptor Antibody (#ADR-004), (1:200).
3,4. Anti-D4 Dopamine Receptor Antibody, preincubated with D4 Dopamine Receptor Blocking Peptide (#BLP-DR004).
- (C)RRWEAARHTKLHSR, corresponding to amino acid residues 215-228 of rat DRD4 (Accession P30729).