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Certificate of Analysis

Dopamine Receptor Antibody Explorer Kit

A Screening Package of Dopamine Receptor Antibodies Economically Priced

Cat #: AK-102
Size: 6 Vials
Form: Lyophilized

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-D1 Dopamine Receptor Antibody Cat #: ADR-001

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IH, WB Reactivity: M, R
Immunogen
Peptide SSHHEPRGSISKDC, corresponding to amino acid residues 372-385 of rat DRD1 (Accession P18901). Intracellular, C-terminus.
Homology
Mouse, dog, pig - identical; human - 13/14 amino acid residues identical.

Guinea pig Anti-D1 Dopamine Receptor Antibody Cat #: AGP-100

Size: 50 µl Host: Guinea pig Type: Polyclonal Application: IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide SSHHEPRGSISKDC, corresponding to amino acid residues 372-385 of rat DRD1 (Accession P18901). Intracellular, C-terminus.
Homology
Mouse, dog, pig - identical; human - 13/14 amino acid residues identical.

Anti-D2 Dopamine Receptor (extracellular) Antibody Cat #: ADR-002

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IH, WB Reactivity: M, R
Immunogen
Peptide (C)DDLERQNWSRPFNGSE, corresponding to amino acid residues 11-26 of rat DRD2 (Accession P61169). Extracellular, N-terminus.
Homology
Mouse - identical; human - 15/16 amino acid residues identical.

Anti-D3 Dopamine Receptor (extracellular) Antibody Cat #: ADR-003

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IFC, IH, WB Reactivity: H, M, R
Immunogen
Peptide CGAENSTGVNRARPH, corresponding to amino acid residues 15-29 of rat DRD3 (Accession P19020). Extracellular, N-terminus.
Homology
Mouse - identical; human - 12/15 amino acid residues identical.

Anti-D4 Dopamine Receptor Antibody Cat #: ADR-004

Size: 50 µl Host: Rabbit Type: Polyclonal Application: WB Reactivity: M, R
Immunogen
Peptide (C)RRWEAARHTKLHSR, corresponding to amino acid residues 215-228 of rat DRD4 (Accession P30729). 3rd intracellular loop.
Homology
Mouse - identical; human - 9/14 amino acid residues identical.

Anti-D5 Dopamine Receptor (extracellular) Antibody Cat #: ADR-005

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IH, WB Reactivity: M, R
Immunogen
Peptide EEGWELEGRTENC, corresponding to amino acid residues 199-211 of rat DRD5 (Accession P25115). 2nd extracellular loop.
Homology
Mouse - 12/13 amino acid residues identical.

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.

Note
A guinea pig polyclonal antibody (#AGP-100) is included in this Explorer Kit. Please take into account when reacting with a secondary antibody.
For research purposes only, not for human use
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