Anti-D2 Dopamine Receptor (extracellular)-FITC Antibody

DRD2, D(2) dopamine receptor
    Cat #: ADR-002-F
    Alternative Name DRD2, D(2) dopamine receptor
  • Lyophilized Powder
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    • Peptide (C)DDLERQNWSRPFNGSE, corresponding to amino acid residues 11-26 of rat DRD2 (Accession P61169). Extracellular, N-terminus.
    • Anti-D2 Dopamine Receptor (extracellular)-FITC Antibody
    Accession (Uniprot) Number P61169
    Gene ID 24318
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - identical; human - 15/16 amino acid residues identical.
    RRID AB_2756583.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Label Fluorescein isothiocyanate (FITC).
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl double distilled water (DDW).
    Antibody concentration after reconstitution 1 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
    Standard quality control of each lot Western blot analysis (unlabeled antibody, #ADR-002), and direct flow cytometry (labeled antibody).
    Applications: fc
    May also work in: ic*, lci*
    Direct flow cytometry
    • Anti-D2 Dopamine Receptor (extracellular)-FITC Antibody
      Cell surface detection of D2 dopamine receptor in live intact human Jurkat T-cell leukemia cell line:
      ___ Cells.
      ___ Cells + Rabbit IgG isotype control-FITC.
      ___ Cells + Anti-D2 Dopamine Receptor (extracellular)-FITC Antibody (#ADR-002-F), (5 µg antibody/0.5x106 cells).
    • Anti-D2 Dopamine Receptor (extracellular)-FITC Antibody
      Cell surface detection of D2 dopamine receptor in live intact mouse M1 myeloid leukemia cells:
      ___ Cells.
      ___ Cells + Rabbit IgG isotype control-FITC.
      ___ Cells + Anti-D2 Dopamine Receptor (extracellular)-FITC Antibody (#ADR-002-F), (10 µg antibody/0.5x106 cells).
    1. Missale, C. et al. (1998) Physiol. Rev. 78, 189.
    2. Kehne, J.H. et al. (2008) Curr. Top. Med. Chem. 8, 1068.
    Scientific background

    The D2 dopamine receptor (DRD2) is one of five receptors that mediate the effects of the catecholamine neurotransmitter dopamine. Dopamine regulates a variety of functions including locomotor activity, emotion, positive reinforcement, food intake, and endocrine regulation. The dopaminergic system has been extensively studied in the last thirty years mainly because its dysregulation has been linked to several neurological and neuropsychiatric diseases including Parkinson’s disease and schizophrenia.1

    All five dopamine receptors belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.

    Historically, the five receptors have been divided into two subfamilies based on pharmacological and structural considerations: the D1-like subfamily (that includes the D1 and D5 subtypes) and the D2-like subfamily (that includes the D2-, D3- and D4 subtypes).1

    The D1-like receptors are coupled to Gs-type G proteins and enhance adenylate cyclase activity while the D2-like receptors are coupled to Gi-type G proteins and inhibit adenylate cyclase activity.1

    D2 receptor expression in the brain is largely confined to the striatum, prefrontal cortex and hypothalamus.1

    Dysregulation of D2 receptor function has been implicated in several disorders including schizophrenia, bipolar disorder, Parkinson's disease, and restless legs syndrome. Consistent with this, treatments that either block or activate the D2 receptor have been developed to treat these diseases.2

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 11/06/2020

    Anti-D2 Dopamine Receptor (extracellular) Antibody (#ADR-002) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize DRD2 from rat, human, and mouse samples.

    Anti-D2 Dopamine Receptor (extracellular)-FITC Antibody (#ADR-002-F) is directly conjugated to fluorescein isothiocyanate (FITC). This labeled antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

    For research purposes only, not for human use
    Shipping and Ordering information