EAAT3/EAAC1 Blocking Peptide (#BLP-GC023) is the original antigen used for immunization during Anti-EAAT3 (EAAC1) Antibody (#AGC-023) generation. The blocking peptide binds and ‘blocks’ Anti-EAAT3/EAAC1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain membranes:
- Western blot analysis of mouse kidney lysate:
- Expression of EAAT3 in rat cerebellumImmunohistochemical staining of perfusion-fixed free-floating frozen rat brain sections with Anti-EAAT3 (EAAC1) Antibody (#AGC-023). A. EAAT3 staining (green) appears in the molecular layer (Mol) and around the soma of Purkinje cells (arrow). B. Parvalbumin (red), a marker of Purkinje and interneuronal cells, stains the same section. C. Merge of the images demonstrates expression of EAAT3 around the soma of Purkinje cells. DAPI is used as the counterstain.
- (C)EPTILDNEDSDTKK, corresponding to amino acid residues 487-500 of rat EAAT3 (Accession P51907).