Overview
Type: Synthetic peptide
Form: Lyophilized powder
EAAT3/EAAC1 Blocking Peptide (#BLP-GC023) is the original antigen used for immunization during Anti-EAAT3 (EAAC1) Antibody (#AGC-023) generation. The blocking peptide binds and ‘blocks’ Anti-EAAT3/EAAC1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-EAAT3 (EAAC1) Antibody (#AGC-023), (1:400).
2. Anti-EAAT3 (EAAC1) Antibody, preincubated with EAAT3/EAAC1 Blocking Peptide (#BLP-GC023).
Western blot analysis of mouse kidney lysate:1. Anti-EAAT3 (EAAC1) Antibody (#AGC-023), (1:400).
2. Anti-EAAT3 (EAAC1) Antibody, preincubated with EAAT3/EAAC1 Blocking Peptide (#BLP-GC023).
Expression of EAAT3 in rat cerebellumImmunohistochemical staining of perfusion-fixed free-floating frozen rat brain sections with Anti-EAAT3 (EAAC1) Antibody (#AGC-023). A. EAAT3 staining (green) appears in the molecular layer (Mol) and around the soma of Purkinje cells (arrow). B. Parvalbumin (red), a marker of Purkinje and interneuronal cells, stains the same section. C. Merge of the images demonstrates expression of EAAT3 around the soma of Purkinje cells. DAPI is used as the counterstain.
Properties
Sequence
- (C)EPTILDNEDSDTKK, corresponding to amino acid residues 487-500 of rat EAAT3 (Accession P51907).
Accession (Uniprot) Number P51907
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
