EphB4 (extracellular) Blocking Peptide (#BLP-ER024) is the original antigen used for immunization during Anti-EphB4 (extracellular) Antibody (#AER-024) generation. The blocking peptide binds and ‘blocks’ Anti-EphB4 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:
- Western blot analysis of human THP-1 monocytic leukemia cell line lysate (lanes 1 and 4), human Jurkat T-cell leukemia cell line lysate (lanes 2 and 5) and human MCF-7 breast adenocarcinoma cell line lysate (lanes 3 and 6):
- Expression of EphB4 in rat hippocampusImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-EphB4 (extracellular) Antibody (#AER-024), (1:100), followed by goat anti-rabbit-AlexaFluor-488. EphB4 immunoreactivity (green) in the hippocampal dentate gyrus is detected in the granule layer (G) and hilar interneurons (arrows). Cell nuclei are stained with DAPI (blue).
- (C)HEKGAEGPSSVRFLK, corresponding to amino acid residues 472-486 of mouse EphB4 (Accession P54761).