Ephrin-A2 (extracellular) Blocking Peptide (#BLP-ER032) is the original antigen used for immunization during Anti-Ephrin-A2 (extracellular) Antibody (#AER-032) generation. The blocking peptide binds and ‘blocks’ Anti-Ephrin-A2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:
- Expression of Ephrin-A2 in mouse hippocampusImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Ephrin-A2 (extracellular) Antibody (#AER-032), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. Ephrin-A2 staining (green) in hippocampal dentate gyrus appears in neurons (vertical arrows) and astrocytes (horizontal arrows). Cell nuclei are stained with DAPI (blue).
- (C)HYGAPLPPAERMER, corresponding to amino acid residues 71-84 of mouse Ephrin-A2 (Accession P52801).