Overview
Type: Synthetic peptide
Form: Lyophilized powder
Ferroportin/SLC40A1 Blocking Peptide (#BLP-IT001) is the original antigen used for immunization during Anti-Ferroportin (SLC40A1) Antibody (#AIT-001) generation. The blocking peptide binds and ‘blocks’ Anti-Ferroportin/SLC40A1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat spleen (lanes 1 and 5), rat small intestine (lanes 2 and 6), rat liver (lanes 3 and 7) and mouse liver (lanes 4 and 8) lysates.1-4. Anti-Ferroportin (SLC40A1) Antibody (#AIT-001), (1:500).
5-8. Anti-Ferroportin (SLC40A1) Antibody, preincubated with Ferroportin/SLC40A1 Blocking Peptide (#BLP-IT001).
Expression of Ferroportin in mouse brainImmunohistochemical staining of mouse cerebellum and forebrain using Anti-Ferroportin (SLC40A1) Antibody (#AIT-001). A. In the cerebellum, Ferroportin staining (green) appears in cerebellar purkinje cells (horizontal arrows) and their dendrites (vertical arrows). B. In the forebrain, intense Ferroportin staining appears (green) in oligodendrocytes (horizontal arrows) and moderate staining is detected in neurons (vertical arrow). In both panels (A and B) DAPI is used as the counterstain.
Expression of Ferroportin in mouse brainImmunohistochemical staining of perfusion-fixed frozen mouse substantia nigra pars compacta (SNC) sections using Anti-Ferroportin (SLC40A1) Antibody (#AIT-001). A. Ferroportin staining (green) appears in several cells in the SNC. B. Tyrosine hydroxylase (red) stains dopaminergic neurons. C. Merge of A and B demonstrates Ferroportin in dopaminergic and non-dopaminergic neurons. Cell nuclei are stained using DAPI (blue).
Properties
Sequence
- (C)EGTHLMGVKDSNIHE, corresponding to amino acid residues 261-275 of human Ferroportin (Accession Q9NP59).
Accession (Uniprot) Number Q9NP59
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
