Ferroportin/SLC40A1 Blocking Peptide (#BLP-IT001) is the original antigen used for immunization during Anti-Ferroportin (SLC40A1) Antibody (#AIT-001) generation. The blocking peptide binds and ‘blocks’ Anti-Ferroportin/SLC40A1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat spleen (lanes 1 and 5), rat small intestine (lanes 2 and 6), rat liver (lanes 3 and 7) and mouse liver (lanes 4 and 8) lysates.
- Expression of Ferroportin in mouse brainImmunohistochemical staining of mouse cerebellum and forebrain using Anti-Ferroportin (SLC40A1) Antibody (#AIT-001). A. In the cerebellum, Ferroportin staining (green) appears in cerebellar purkinje cells (horizontal arrows) and their dendrites (vertical arrows). B. In the forebrain, intense Ferroportin staining appears (green) in oligodendrocytes (horizontal arrows) and moderate staining is detected in neurons (vertical arrow). In both panels (A and B) DAPI is used as the counterstain.
- Expression of Ferroportin in mouse brainImmunohistochemical staining of perfusion-fixed frozen mouse substantia nigra pars compacta (SNC) sections using Anti-Ferroportin (SLC40A1) Antibody (#AIT-001). A. Ferroportin staining (green) appears in several cells in the SNC. B. Tyrosine hydroxylase (red) stains dopaminergic neurons. C. Merge of A and B demonstrates Ferroportin in dopaminergic and non-dopaminergic neurons. Cell nuclei are stained using DAPI (blue).
- (C)EGTHLMGVKDSNIHE, corresponding to amino acid residues 261-275 of human Ferroportin (Accession Q9NP59).