Cat #: BLP-FR023
Type: Synthetic peptide
Form: Lyophilized Powder
A Blocking peptide for Anti-Frizzled-3 (FZD3) Antibody (#AFR-023). Intended to be used as a negative control.
For research purposes only, not for human use
- Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
- Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:
- Expression of Frizzled-3 in mouse striatum.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Frizzled-3 (FZD3) Antibody (#AFR-023), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Frizzled-3 immunoreactivity (green) appears in neuronal profiles (arrows). B. Pre-incubation of the antibody with Frizzled-3 (FZD3) Blocking Peptide (BLP-FR023), suppressed staining. Cell nuclei are stained with DAPI (blue).
- Peptide (C)KVSSYHGSLHRSRDGR, corresponding to amino acid residues 581 - 596 of mouse Frizzled-3 (Accession Q61086).
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
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