Frizzled-3 (FZD3) Blocking Peptide (#BLP-FR023) is the original antigen used for immunization during Anti-Frizzled-3 (FZD3) Antibody (#AFR-023) generation. The blocking peptide binds and ‘blocks’ Anti-Frizzled-3 (FZD3) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
- Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:
- Expression of Frizzled-3 in mouse striatum.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Frizzled-3 (FZD3) Antibody (#AFR-023), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Frizzled-3 immunoreactivity (green) appears in neuronal profiles (arrows). B. Pre-incubation of the antibody with Frizzled-3 (FZD3) Blocking Peptide (BLP-FR023), suppressed staining. Cell nuclei are stained with DAPI (blue).
- Peptide (C)KVSSYHGSLHRSRDGR, corresponding to amino acid residues 581 - 596 of mouse Frizzled-3 (Accession Q61086).