GABA(A) α1 Receptor (extracellular) Blocking Peptide

1. Anti-GABA(A) α1 Receptor (extracellular) Antibody (#AGA-001), (1:1000).
2. Anti-GABA (A) α1 Receptor (extracellular) Antibody, preincubated with GABA(A) α1 Receptor (extracellular) Blocking Peptide (#BLP-GA001).

Immunohistochemical staining of mouse hippocampus using Anti-GABA(A) α1 Receptor (extracellular) Antibody (#AGA-001). A. Distribution of GABRA1 (red). B. Distribution of glial fibrillary acidic protein (green). C. Merge of the two images indicates that distribution of GABRA1 is restricted to neurons and their processes. DAPI is used as the counterstain (blue).

Immunohistochemical staining of rat cerebellum using Guinea pig Anti-Ca_V1.2 (CACNA1C) Antibody (#AGP-001) and Anti-GABA(A) α1 Receptor (extracellular) Antibody (#AGA-001). A. Ca_V1.2 (green) is detected in the granule layer of the cerebellum (G) and in the upper molecular layer (star). B. In the same section, GABRA1 (red) is seen in the granule layer. C. Merge of the two images reveals high degree of colocalization between Ca_V1.2 and GABRA1 in the granule layer.

Immunohistochemical staining of mouse and rat hippocampal dentate gyrus using Guinea pig Anti-Ca_V1.2 (CACNA1C) Antibody (#AGP-001) and Anti-GABA(A) α1 Receptor (extracellular)-ATTO Fluor-488 Antibody (#AGA-001-AG) in the same section. Both Ca_V1.2 (red) and GABRA1 (green) are detected in neuron-shaped cells (arrows). Staining suggests partial colocalization between Ca_V1.2 and GABRA1 in a sub-population of rat dentate gyrus neurons. A. Mouse hippocampus. B. Rat hippocampus.

Immunohistochemical staining of rat dentate gyrus using Anti-GABA(A) α1 Receptor (extracellular)-ATTO Fluor-488 Antibody (#AGA-001-AG) (green). Staining reveals hilar neurons (arrows) and fibers coursing through the granule layer (G). DAPI is used as the counterstain (blue).

1-3. Guinea pig Anti-GABA(A) α1 Receptor (extracellular) Antibody (#AGP-083), (1:400).
4-6. Guinea pig Anti-GABA(A) α1 Receptor (extracellular) Antibody, preincubated with GABA(A) α1 Receptor (extracellular) Blocking Peptide (#BLP-GA001).

Following a broad screen of secondary antibodies, the following were used for this application:
Western blot analysis:
#106-035-006 (Jackson ImmunoResearch) 
#A7289 (Sigma)

Immunohistochemical staining of perfusion-fixed frozen rat brain sections using rabbit Anti-KCC2 (SLC12A5) Antibody (#AKT-005), (1:400) and Guinea pig Anti-GABA(A) α1 Receptor (extracellular) Antibody (#AGP-083), (1:400). A. KCC2 immunoreactivity (green) is detected in both the granule layer (GL) and the molecular layer (MOL). B. GABA(A) α1 receptor staining (red) is observed mainly in the granule layer (GL). C. Merge of the two images demonstrates colocalization in the granule layer (arrows). Cell nuclei are stained with DAPI (blue).

Immunohistochemical staining of rat hippocampal dentate gyrus sections using Guinea pig Anti-GABA(A) α1 Receptor (extracellular) Antibody (#AGP-083). A. GABRA1 staining (red) appears in neuronal soma (horizontal arrows in panel B) and in the neuronal processes (vertical arrows in panel B). B. GABRA1 staining merged with DAPI counterstain (blue).