GABA(A) γ1 Receptor Blocking Peptide (#BLP-GA016) is the original antigen used for immunization during Anti-GABA(A) γ1 Receptor Antibody (#AGA-016) generation. The blocking peptide binds and ‘blocks’ Anti-GABA(A) γ1 Receptor primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
- Expression of GABA(A) γ1 receptor in rat DRGImmunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-GABA(A) γ1 Receptor Antibody (#AGA-016), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. A. Staining (red) appears in neuronal cell bodies. B. Nuclear staining using DAPI as the counterstain. C. Merged image of A and B.
- (C)HAGSTLIPMNNISMPQGE, corresponding to amino acid residues 382-399 of rat GABA(A) γ1 receptor (Accession P23574).