GALR3 (extracellular) Blocking Peptide (#BLP-GR013) is the original antigen used for immunization during Anti-GALR3 (extracellular) Antibody (#AGR-013) generation. The blocking peptide binds and ‘blocks’ Anti-GALR3 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain membrane:
- Expression of Galanin receptor 3 in rat hippocampusImmunohistochemical staining of rat hippocampus using Anti-GALR3 (extracellular) Antibody (#AGR-013). A. Galanin receptor 3 (green) appears in apical dendrites of pyramidal neurons in CA1 (arrows). B. MAP2 (red), a marker of axons and dendrites, is stained in the same section. GALR3 appears to be expressed in a subset of apical dendrites (arrows). DAPI is used as the counterstain (blue).
- SYYGTVRYGALELC, corresponding to amino acid residues 159-172 of mouse Galanin receptor type 3 (Accession O88853).