GAP43 Blocking Peptide (#BLP-NR016) is the original antigen used for immunization during Anti-GAP43 Antibody (#ANR-016) generation. The blocking peptide binds and ‘blocks’ Anti-GAP43 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:
- Expression of GAP43 in rat hippocampusImmunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-GAP43 Antibody (#ANR-016), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. A. GAP43 staining (green) appears in a layer bordering granule layer (G), (arrow). B. Calbindin D28k staining (red) is detected in granule layer (G) neurons. C. Merge of the two images demonstrates distinct spatial localizations in the hippocampal dentate gyrus. Cell nuclei are stained with DAPI (blue).
- Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
- Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:
- Expression of GAP43 in rat hippocampus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Guinea Pig Anti-GAP43 Antibody (#ANR-016-GP), (1:300), followed by goat anti-guinea pig - Alexa Fluor-594. A. Staining in the hippocampal dentate gyrus region, showed immunoreactivity (red) in the subgranular layer (arrows), over the granule layer (G) and over the outer molecular layer (OML). B. Pre-incubation of the antibody with GAP43 Blocking Peptide (#BLP-NR016), suppressed staining. Cell nuclei are stained with DAPI (blue).
- (C)KPKESARQDEGKED, corresponding to amino acid residues 205-218 of rat GAP43 (Accession P07936).