GDNF Blocking Peptide (#BLP-NT014) is the original antigen used for immunization during Anti-GDNF Antibody (#ANT-014) generation. The blocking peptide binds and ‘blocks’ Anti-GDNF primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of Recombinant human GDNF protein:
- Expression of GDNF in rat dorsal root ganglion (DRG).Immunohistochemical staining of rat DRG longitudinal frozen section using Anti-GDNF Antibody (#ANT-014), (1:50), followed by Alexa 555-conjugated goat anti-rabbit antibody (red). Hoechst 33342 (blue) was used as a counterstain. GDNF staining is specific for neurons. Note that Schwann cells as well as satellite glial cells are both negative.
- Expression of GDNF in rat hippocampusImmunohistochemical staining of rat hippocampus frozen sections with Anti-GDNF Antibody (#ANT-014), (1:100), (red). A. GDNF staining is apparent in the pyramidal layer (asterik), in astrocytic fibers (horizontal arrows) and in some interneurons (vertical arrow). B. Calbindin D28k (green) appears in a subset of cells in the pyramidal layer. C. Merge of GDNF and calbindin demonstrates some co-localization in neurons. DAPI is used as the counterstain (blue).
- (C)DDNLVYHILRKHSAKR, corresponding to amino acid residues 192-207 of human GDNF (precursor) (Accession P39905).