GIPC1 Blocking Peptide (#BLP-PZ045) is the original antigen used for immunization during Anti-GIPC1 Antibody (#APZ-045) generation. The blocking peptide binds and ‘blocks’ Anti-GIPC1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
- Western blot analysis of human U87-MG glioblastoma cell line lysate:
- Expression of GIPC1 in mouse and rat hippocampusImmunohistochemical staining of perfusion-fixed frozen mouse and rat brain sections with Anti-GIPC1 Antibody (#APZ-045), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. A. In mouse brain GIPC1 staining (green) appears in neurons of the pyramidal layer (P) and their apical dendrites (arrows). B. In rat brain similar staining is observed. Cell nuclei are stained with DAPI (blue).
- (C)KAPPLVENEEAEPGR, corresponding to amino acid residues 10-24 of human GIPC1 (Accession O14908).