Cat #: BLP-GT021
Type: Synthetic peptide
Form: Lyophilized powder
A Blocking peptide for Anti-GLUT1 Antibody (#AGT-021). Intended to be used as a negative control.
For research purposes only, not for human use
- Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
- Expression of Glucose Transporter 1 in mouse striatum and wall of lateral ventricleImmunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-GLUT1 Antibody (#AGT-021), (1:400), followed by donkey-anti-rabbit-Cy3 secondary antibody (red). GLUT1 staining appears in blood vessels (right-pointing arrows) in the striatum (ST) and in the wall of the lateral ventricle (LV, left-pointing arrow). Cell nuclei were stained with DAPI (blue).
- (C)RQGGASQSDKTPEE, corresponding to amino acid residues 468-481 of human Glucose transporter 1 (Accession P11166).
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 3 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
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