Overview
Type: Synthetic peptide
Form: Lyophilized powder
GLUT1 Blocking Peptide (#BLP-GT021) is the original antigen used for immunization during Anti-GLUT1 Antibody (#AGT-021) generation. The blocking peptide binds and ‘blocks’ Anti-GLUT1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only, not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):1, 2. Anti-GLUT1 Antibody (#AGT-021), (1:500).
3, 4. Anti-GLUT1 Antibody, preincubated with GLUT1 Blocking Peptide (#BLP-GT021).
Expression of Glucose Transporter 1 in mouse striatum and wall of lateral ventricleImmunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-GLUT1 Antibody (#AGT-021), (1:400), followed by donkey-anti-rabbit-Cy3 secondary antibody (red). GLUT1 staining appears in blood vessels (right-pointing arrows) in the striatum (ST) and in the wall of the lateral ventricle (LV, left-pointing arrow). Cell nuclei were stained with DAPI (blue).
Properties
Sequence
- (C)RQGGASQSDKTPEE, corresponding to amino acid residues 468-481 of human Glucose transporter 1 (Accession P11166).
Accession (Uniprot) Number P11166
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 3 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
