GLUT2/SLC2A2 Blocking Peptide (#BLP-GT022) is the original antigen used for immunization during Anti-GLUT2 (SLC2A2) Antibody (#AGT-022) generation. The blocking peptide binds and ‘blocks’ Anti-GLUT2/SLC2A2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat liver membranes:
- Western blot analysis of mouse brain membranes:
- Western blot analysis of human Caco-2 colorectal adenocarcinoma cell line lysate (lanes 1 and 3) and human HepG2 liver hepatocellular carcinoma cell lysate (lanes 2 and 4):
- Expression of Glucose Transporter 2 in rat cerebellum and hippocampusImmunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-GLUT2 (SLC2A2) Antibody (#AGT-022), (1:120), followed by goat-anti-rabbit-AlexaFluor-488 (green). Cell nuclei are stained with DAPI (blue). A. Staining in cerebellum sections, reveals GLUT2 labeling in Purkinje cells (arrows). B. Staining in hippocampus dentate gyrus, shows labeling of both granule cells (horizontal arrow) and in hilar interneurons (vertical arrows).
- (C)EFRKKSGSAPPRK, corresponding to amino acid residues 496-508 of rat Glucose Transporter 2 (Accession P12336).