GnRH Receptor (extracellular) Blocking Peptide (#BLP-GR030) is the original antigen used for immunization during Anti-GnRH Receptor (extracellular) Antibody (#AGR-030) generation. The blocking peptide binds and ‘blocks’ Anti-GnRH Receptor (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat ovary (lanes 1 and 3) and pituitary (lanes 2 and 4) lysates:
- Expression of GnRHR in rat ovaryImmunohistochemical staining of rat ovary paraffin-embedded sections using Anti-GnRH Receptor (extracellular) Antibody (#AGR-030). GnRHR labeling appears in the stroma surrounding the follicles. The stroma is a richly cellular connective tissue with scattered smooth muscle fibers. The theca layer is stained as well. Hematoxilin is used as the counterstain.
- (C)NNASLEQDQNH, corresponding to amino acid residues 3-13 of rat GnRHR (Accession P30969).