GPR12 (extracellular) Blocking Peptide (#BLP-GR062) is the original antigen used for immunization during Anti-GPR12 (extracellular) Antibody (#AGR-062) generation. The blocking peptide binds and ‘blocks’ Anti-GPR12 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain lysates (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):1-2. Anti-GPR12 (extracellular) Antibody (#AGR-062), (1:200).
3-4. Anti-GPR12 (extracellular) Antibody, preincubated with GPR12 (extracellular) Blocking Peptide (BLP-GR062).
- Expression of GPR12 in rat cortex.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-GPR12 (extracellular) Antibody (#AGR-062), (1:300), followed by donkey anti-rabbit-biotin and Streptavidin-Cy3. A. Staining in the parietal cortex region, showed GPR12 immunoreactivity (red) in glial cell outlines (arrows). B. Pre-incubation of the antibody with GPR12 (extracellular) Blocking Peptide (BLP-GR062), suppressed staining. Cell nuclei are stained with DAPI (blue).
- Peptide (C)EDPKVNLSGLPRD, corresponding to amino acid residues 3 - 15 of mouse GPR12 (Accession P35412).