GPR120/FFAR4 (extracellular) Blocking Peptide (#BLP-FR014) is the original antigen used for immunization during Anti-GPR120/FFAR4 (extracellular) Antibody (#AFR-014) generation. The blocking peptide binds and ‘blocks’ Anti-GPR120/FFAR4 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat lung (lanes 1 and 3), rat tongue (lanes 2 and 4), human U-87 astrocytoma cells (lanes 5 and 7) and mouse MS1 endothelial cells (lanes 6 and 8) lysates:
- Western blot analysis of mouse colon (lanes 9 and 11) and human colorectal adenocarcinoma (Colo 205) cell line (lanes 10 and 12) lysates:
- Expression of FFAR4 in rat hypothalamus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-GPR120/FFAR4 (extracellular) Antibody (#AFR-014), (1:1200), followed by biotinylated donkey anti-rabbit and streptavidin-Cy3. A. FFAR4 immunoreactivity (red) appears in axons beaded with varicosities (arrows). . B. Pre-incubation of the antibody with GPR120/FFAR4 (extracellular) Blocking Peptide (BLP-FR014), suppressed staining. Cell nuclei are stained with DAPI (blue). VMH = ventromedial hypothalamus, 3V = 3rd ventricle
- (C)RTHFPFFSDVKGDHR, corresponding to amino acid residues 22-36 of mouse FFAR4 (Accession Q7TMA4).