GPR142 (extracellular) Blocking Peptide (#BLP-GR082) is the original antigen used for immunization during Anti-GPR142 (extracellular) Antibody (#AGR-082) generation. The blocking peptide binds and ‘blocks’ Anti-GPR142 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse pancreas lysate (lanes 1 and 3) and rat pancreas lysate (lanes 2 and 4):
- Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):
- Western blot analysis of human THP-1 monocytic leukemia cell line lysate (lanes 1 and 4), human Jurkat T-cell leukemia cell line lysate (lanes 2 and 5) and human SH-SY5Y neuroblastoma cell line lysate (lanes 3 and 6):
- Expression of GPR142 in rat ventromedial hypothalamus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-GPR142 (extracellular) Antibody (#AGR-082), (1:1000), followed by goat anti-rabbit-AlexaFluor-488. A. GPR142 immunoreactivity (green) appears in glial processes (vertical arrow) and in cells lining the wall of 3rd ventricle (horizontal arrow). B. Pre-incubation of the antibody with GPR142 (extracellular) Blocking Peptide (BLP-GR082), suppressed staining. Cell nuclei are stained with DAPI (blue). 3rd V= 3rd ventricle.
- Peptide (C)APVHRDWRVHLALD, corresponding to amino acid residues 291 - 304 of mouse GPR142 (Accession Q7TQN9).