GPR174 (extracellular) Blocking Peptide (#BLP-GR060) is the original antigen used for immunization during Anti-GPR174 (extracellular) Antibody (#AGR-060) generation. The blocking peptide binds and ‘blocks’ Anti-GPR174 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain membranes (lanes 1 and 5), mouse brain lysate (lanes 2 and 6), rat spleen lysate (lanes 3 and 7) and mouse spleen membranes (lanes 4 and 8):
- Western blot analysis of human MOLT-4 T-cell leukemia cell line lysate (lanes 1 and 3) and human Jurkat T-cell leukemia cell line lysate (lanes 2 and 4):
- Peptide (C)DTSGNRTK(S)FVDLPTR, corresponding to amino acid residues 160 - 175 of human GPR174 (Accession Q9BXC1).