GPR34 (extracellular) Blocking Peptide (#BLP-GR055) is the original antigen used for immunization during Anti-GPR34 (extracellular) Antibody (#AGR-055) generation. The blocking peptide binds and ‘blocks’ Anti-GPR34 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
- Western blot analysis of mouse BV-2 microglia cell line lysate (lanes 1 and 3) and human THP-1 monocytic leukemia cell line lysate (lanes 2 and 4):
- CFHYRDRHNAKGE, corresponding to amino acid residues 197 - 209 of mouse GPR34 (Accession Q9R1K6).