GPR50 Blocking Peptide (#BLP-GR061) is the original antigen used for immunization during Anti-GPR50 Antibody (#AGR-061) generation. The blocking peptide binds and ‘blocks’ Anti-GPR50 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):1-2. Anti-GPR50 Antibody (#AGR-061), (1:200).
3-4. Anti-GPR50 Antibody, preincubated with GPR50 Blocking Peptide (BLP-GR061).
- Peptide (C)ESFRREYWTIFHAMR, corresponding to amino acid residues 307 - 321 of mouse GPR50 (Accession O88495).