Overview
Type: Synthetic peptide
Form: Lyophilized Powder
GPR97 (ADGRG3) (extracellular) Blocking Peptide (#BLP-GR057) is the original antigen used for immunization during Anti-GPR97 (ADGRG3)(extracellular) Antibody (#AGR-057) generation. The blocking peptide binds and ‘blocks’ Anti-GPR97 (ADGRG3) (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):1-2. Anti-GPR97 (ADGRG3)(extracellular) Antibody (#AGR-057), (1:200).
3-4. Anti-GPR97 (ADGRG3)(extracellular) Antibody, preincubated with GPR97 (ADGRG3)(extracellular) Blocking Peptide (BLP-GR057).
Western blot analysis of human THP-1 monocytic leukemia cell line lysate:1. Anti-GPR97 (ADGRG3)(extracellular) Antibody (#AGR-057), (1:200).
2. Anti-GPR97 (ADGRG3)(extracellular) Antibody, preincubated with GPR97 (ADGRG3)(extracellular) Blocking Peptide (BLP-GR057).
Expression of GPR97 in rat spinal cord.Immunohistochemical staining of perfusion-fixed frozen rat spinal cord sections with Anti-GPR97 (ADGRG3)(extracellular) Antibody (#AGR-057), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the ventral horn of the spinal cord region, showed immunoreactivity of GPR97 (green) in neuronal outlines (arrows). B. Pre-incubation of the antibody with GPR97 (ADGRG3)(extracellular) Blocking Peptide (BLP-GR057), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide CNVENLQRYWLNYE, corresponding to amino acid residues 62 - 75 of human ADGRG3 (Accession Q86Y34).
Accession (Uniprot) Number Q86Y34
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
