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Guinea pig Anti-P2X4 Receptor Antibody

P2RX4, P2X purinoceptor 4, ATP receptor
Cat #: AGP-085
Alternative Name P2RX4, P2X purinoceptor 4, ATP receptor
Lyophilized Powder yes
Type: Polyclonal
Host: Guinea Pig
Reactivity: h, m, r
Immunogen
  • Peptide (C)KKYKYVEDYEQGLSGEMNQ, corresponding to amino acid residues 370-388 of rat P2X4 receptor (Accession P51577). Intracellular, C-terminus.
Accession (Uniprot) Number P51577
Gene ID 29659
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - identical; human - 15/19 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Guinea pig total IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*
Western blot
  • Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and human U-87 MG glioblastoma cell (lanes 3 and 6) lysates:
    Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and human U-87 MG glioblastoma cell (lanes 3 and 6) lysates:
    1,2. Guinea pig Anti-P2X4 Receptor Antibody (#AGP-085), (1:500).
    3.    Guinea pig Anti-P2X4 Receptor Antibody (1:1000).
    4-6. Guinea pig Anti-P2X4 Receptor Antibody, preincubated with P2X4 Receptor Blocking Peptide (#BLP-PR002).
References
  1. Prasad, M. et al. (2001) J. Physiol. 537, 667.
  2. Florenzano, F. et al. (2002) Neuroscience 115, 425.
  3. Ashcroft, F.M. et al. (2000) Ion Channels and Disease Ed 1, p. 405, Academic Press, San Diego.
  4. Khakh, B.S. et al. (2001) Pharmacol Rev. 53, 107.
  5. Ding, Y. et al. (2000) J. Auton. Nerv. Syst. 81, 289.
  6. Le, K.T. et al. (1998) J. Neurosci. 18, 7152.
  7. Robertson, S.J. et al. (2001) Curr. Opin. Neurobiol. 11, 378.
  8. Dunn, P.M. et al. (2001) Prog. Neurobiol. 65, 107.
  9. Kim, M. et al. (2001) EMBO J. 20, 6347.
  10. Inoue, K. et al. (2004) J. Pharmacol. Sci. 94, 112.
Scientific background

The P2X purinergic receptors belong to the ligand-gated ion channel family and are activated by extracellular ATP.

The structure and function of the P2X receptors, which were mainly investigated using in vitro models, indicate their involvement in synaptic communication, cell death, and differentiation.

Seven mammalian P2X receptor subtypes (P2X1–P2X7) have been identified and cloned.1,2,3 All P2X receptor subtypes share the same structure of intracellular N- and C-termini, two membrane-spanning domains and a large extracellular loop.

All P2X subunits can assemble to form homomeric or heteromeric functional channels with the exception of P2X6, which only appears to function as part of a heteromeric complex.4-9

The various P2X receptors show distinct expression patterns. P2X1-6 have been found in the central and peripheral nervous system, while the P2X7 receptor is predominantly found in cells of the immune system.4

The P2X2 receptor subunit has a widespread tissue distribution in autonomic neurons, but it is generally found to be co-expressed with one or more subtypes.

Overexpression of P2X4 was demonstrated in microglia and in the spinal dorsal horn following peripheral nerve injury. It has been suggested that activation of P2X4 along with p38 MAPK is essential for the development of allodynia (pain from a stimulus that doesn't normally elicit pain) following nerve injury. Inhibition of P2X4 expression in spinal microglia has been suggested as a novel therapeutic approach for the treatment of allodynia.10

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 23/08/2020

Guinea pig Anti-P2X4 Receptor Antibody (#AGP-085), raised in guinea pigs, is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize P2X4 from mouse, rat, and human samples. The antigen used to immunize guinea pigs is the same as Anti-P2X4 Receptor Antibody (#APR-002) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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