HCN3 (extracellular) Blocking Peptide (#BLP-PC083) is the original antigen used for immunization during Anti-HCN3 (extracellular) Antibody (#APC-083) generation. The blocking peptide binds and ‘blocks’ Anti-HCN3 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
- (C)ELEPRLDAEVYK, corresponding to amino acid residues 190-201 of rat HCN3 (Accession Q9JKA8).